icon_100_ngs

Adapters for next generation sequencing platforms

High quality adapter products to meet the ever-expanding needs of researchers

Adapters are a key component of the next generation sequencing (NGS) workflow. Each major NGS platform has recommended adapter sequences, which are used to prepare sample libraries. Regardless of the NGS instrument, synthesis scale, or manufacturing quality standard desired, IDT has been serving the needs of NGS researchers from the beginning. We have become a recognized leader in adapters by leveraging our extensive expertise in custom oligo manufacturing and our commitment to quality and our customers.

Enjoy these benefits of getting NGS adapters from IDT:

  • Comprehensive adapter offering
  • Trusted customer support
  • Fast turnaround time

Due to our leadership in NGS adapters, Illumina chose IDT as its partner to develop the next generation of index adapters to improve library preparation multiplexing. These new sets of adapters contain unique dual indexes (UDIs) that mitigate sample misassignment due to index hopping. IDT will manufacture and supply Illumina with the UDI adapters and is also licensed to sell these index sequences as custom UDI-containing adapters. See the press release.

NGS-Adapters-webpage-figure

Adapter design strategies may include single or dual indexes depending on the number of libraries combined prior to sequencing (see diagram above). Poor sequencing data can result from sub-quality adapters either having low purity (i.e., a high proportion of non-full-length adapters) or high cross contamination of other adapter species possibly leading to sample misassignment during analysis.

The use of unique molecular identifiers (UMIs), incorporated into the xGen Duplex Seq and xGen Dual Index UMI adapters, has been shown to reduce the rate of false positive variant calls and increase variant detection sensitivity due to the ability to individually tag each original DNA fragment and identify whether the variant allele was present in the original sample. UMI-containing adapters are available from IDT with a dual index design for low frequency variant detection.