GMP Probes and Primers

Technology you can trust for gene expression and genotyping

Our GMP Oligo Services offer convenient, online ordering of small- to mid-scale synthesis of double-quenched probes and primers used for gene expression and genotyping.

  • Easy online ordering
  • Affordable pricing
  • Expeditious turnaround
  • Flexible probe and primer configuration

As an extension of our PrimeTime® qPCR products for research, our GMP probes and primers enable smooth transition of your product from research to commercialization. 

Contact Us

Email gmpinfo@idtdna.com or call 800-328-2661

GMP probes and primers

Probes

Length: 15–45 bases
Fluorophore options: FAM, Cy®3, Cy5, HEX™, JOE, ROX™, TET™ or Yakima Yellow®
Quencher options: Iowa Black® RQ, Iowa Black FQ, or ZEN™/Iowa Black FQ
Available in a range of deliverable amounts.

New! Yakima Yellow® dye now available!


Primers

Length: 5–60 bases
Available in a range of deliverable amounts

Purification options

Both probes and primers can be HPLC purified

Turnaround time and shipment conditions

Standard desalt products typically ship in 2 weeks.
Purified products typically ship 3 weeks.

Lyophilized products will be shipped under ambient conditions.
Resuspended products will be shipped on dry ice.

From research to clinic

As an extension of IDT’s PrimeTime® portfolio for research applications, our GMP probes and primers enable smooth progression of your product pipeline from research to commercialization.


ZEN™ Double-Quenched Probes

Our proprietary ZEN Double-Quenched Probes offer superior performance compared to traditional single-quenched probes. They display consistently earlier Cq values and improved precision when compared to traditional probes. For more information please download the PrimeTime® Custom qPCR Probes Flyer.

Freedom™ Dyes for affordable diagnostics

We offer dyes with no patent licensing restrictions from IDT or third party companies. Freedom Dyes include FAM, HEX™, JOE, MAX™, TET™, ROX™, TAMRA, and others.  Additionally, ATTO™ Dyes are excellent substitutes for VIC®, LIZ®, and Alexa Fluor® dyes. These dyes are fluorescent markers that feature strong absorption and sensitivity, enhanced photostability, and high fluorescence quantum yield. Learn more at our about our Freedom Dye portfolio. 

Regulatory burdens

To alleviate unknown or undefined regulatory burdens for components in LDTs, IVDs and other applications, we recommend GMP oligos. Our team will help you manage the oligo related regulatory and legal burdens required in the diagnostic space. Our facilities are ISO 13485:2003 certified and compliant with US Food & Drug Administration (FDA) Quality System Regulation (QSR) 21 CFR Part 820, and we maintain an open door audit policy. 

Need an ASR?

Should your work require the use of a labeled analyte-specific reagent, simply choose ASR labeling service for your GMP oligos. Your products will arrive with a Certificate of Analysis (COA) indicating as such, and the supporting GMP paperwork to ensure your documentation requirements are covered.

Genotyping applications

MGB Eclipse® probes and primers are ideal for genotyping analysis by qPCR.

Comparison qPCR studies of multiple MGB™ probe and primer sets manufactured by IDT versus those made by another leading manufacturer demonstrated equivalent genotyping calls for KRAS mutations and high specificity for the appropriate wild-type or mutant allele (Figure 1). End-point fluorescent signal intensities were similar or slightly higher using MGB Eclipse® Probes from IDT.

Comparison Genotyping Data

Figure 1. Genotyping results from KRAS G12R qPCR assays using probe and primers manufactured by IDT and another leading manufacturer. The KRAS G12R qPCR assays used MGB Eclipse® probes (FAM dye—wild-type probe; TET™ dye—mutant probe) and primers made by either IDT or another leading manufacturer (Company L). Reactions (10 µL) were run with 104 copies of wild-type, mutant, or pooled wild-type/mutant template (IDT® gBlocks® Gene Fragments) and TaqMan® Gene Expression Master Mix (Life Technologies) on a CFX384 Touch™ Real-Time PCR Detection System (BioRad). Cycling conditions were 3 min. 95°C; 50 x (10 sec. 95°C, 30 sec. 60°C).

About GMP manufacturing at IDT

Our GMP Manufacturing services include dedicated ordering, production, final-fill, and shipping systems that meet the elevated quality standards required for products used in clinical or molecular diagnostic applications. IDT is a contract GMP manufacturer of oligonucleotide reagent components for in vitro diagnostic devices (IVDs) and analyte-specific reagents (ASRs) for laboratory-developed tests (LDTs). IDT's GMP facilities are:

  • ISO 13485:2003 certified (since 2008)
  • Compliant with US Food & Drug Administration (FDA) Quality System Regulation (QSR) 21 CFR Part 820
  • US FDA registered (3004613294)